The identification of microorganisms with precision is essential
across a range of fields such as medicine, biotechnology, and
ecology. Whole genome sequencing technologies are a powerful tool
for achieving accurate identification, providing more precise
results than traditional 16S rRNA gene sequencing. As a result,
regulatory agencies in Europe and the US now require genomic
characterization of microorganisms for approval in food and
production applications.
TAXON establishes taxonomic identity by ensuring coherence among
phylogenetic and genomic characteristics, which is crucial for
accurate classification and reliable commercial or industrial
applications.
TAXON uses a pipeline for microbe identification that integrates genomic and phylogenomic analyses. First, we calculate Average Nucleotide Identity values and perform complementary analyses such as is- DDH if necessary. Then, core genes using BLAST in all bacterial genomes, followed by individual alignment, concatenation, trimming, selecting the best substitution model, and inferring evolutionary history.
In this case study, we combined analyses of core-based phylogeny
and all versus all Average Nucleotide Identity values based on >
2100 strains to update the genomospecies circumscriptions within
B. cereus group. These analyses suggested the existence
of 57 genomospecies, 37 of which are novel. The figure shows the
phylogenomic tree of Clade 1 of B. cereus group strains.
The tree was constructed with individually aligned core genes.
Reference: Torres Manno, M.A., Repizo, G.D., Magni, C. et al.
The assessment of leading traits in the taxonomy of the
Bacillus cereus group. Antonie van Leeuwenhoek 113,
2223–2242 (2020).
https://doi.org/10.1007/s10482-020-01494-3
Get reliable and comprehensive genomic assembly and annotation tailored to your research needs.
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Identify genes responsible for desirable traits and ensure the absence of undesirable characteristics, enabling the development of safer and more efficient microorganisms.
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Genomic or metagenomic data are searched for genes encoding proteins with exceptional characteristics that can be used for specific industrial, agronomical, or pharmaceutical purposes.
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The whole genomic sequences of various organisms are analyzed and compared through comparative genomics. This helps in determining the uniqueness of the trait of interest.
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