Metatranscriptomic

Metatranscriptomics is the analysis of RNA expression and functions within complex microbial communities. At TAXON, we specialize in advanced bioinformatics approaches to unravel valuable insights into active genes, metabolic pathways, and microbial interactions. Our tailored metatranscriptomics services cater to environmental, industrial, and biomedical researchers, offering comprehensive analysis and interpretation of existing metatranscriptomic data. Partner with us to unlock the hidden potential of microbial communities and drive innovation in your research.

Metatranscriptomics is an advanced field that examines the expression and functional analysis of RNA molecules within complex microbial communities. It provides valuable insights into active genes, metabolic pathways, and microbial interactions that drive community dynamics. At TAXON, we offer comprehensive metatranscriptomics services tailored to meet diverse research objectives.

Our metatranscriptomics services include:

1. Preprocessed and quality-controlled data: We employ robust computational pipelines to preprocess metatranscriptomic data, eliminating sequencing artifacts, filtering low-quality reads, and trimming adapters. This ensures reliable and accurate data for downstream analysis.
2. Transcript assembly and functional annotation: Using specialized algorithms, we reconstruct and annotate transcripts from metatranscriptomic data, identifying genes and associated functions. This sheds light on the metabolic potential and functional diversity of microbial communities.
3. Differential gene expression analysis: Statistical methods are utilized to compare gene expression levels across different conditions or time points within microbial communities. This analysis uncovers key genes and pathways actively responding to environmental changes or community interactions.
4. Identification of genes or enzymes with desirable characteristics: Our analysis can provide a list of genes or enzymes exhibiting specific desirable traits, such as temperature resistance or specific activity. This information is valuable for identifying enzymes with high practical value for industrial applications.

Our metatranscriptomics services empower researchers to unravel the functional dynamics of microbial communities and gain deeper insights into their roles across various ecosystems. Whether studying environmental samples, host-microbiome interactions, or industrial processes, TAXON is committed to delivering high-quality metatranscriptomics analyses tailored to your specific research goals.

Deliverables:

  • Tables of differential gene expression: Detailed tables showcasing genes with significant expression changes between different conditions or sample groups. Includes expression levels, statistical scores, and direction of expression changes.
  • Plots depicting differential expression: Visual representations (e.g., bar graphs, scatter plots, heatmaps) illustrating gene expression profiles. Facilitates identification of the most relevant genes in terms of expression alterations.
  • Functional annotation of genes: Tables or files containing information about the biological function and associated metabolic pathways of identified genes. Utilizes databases such as Gene Ontology, KEGG, or COG for annotations.
  • Protein sequences: Files providing translated protein sequences derived from identified genes. Useful for further investigations related to protein function and characteristics.
  • Transcriptome assembly files: If transcriptome assembly was performed, these files contain reconstructed transcript sequences in formats like FASTA or GFF.
  • Metadata files: Additional files containing contextual information about the samples, such as experimental conditions, geographic location, or environmental factors. Essential for result interpretation and data contextualization.
  • Please note that specific deliverables may vary depending on the service provider or software used. For more detailed information on the specific deliverables offered by TAXON, please contact us directly or refer to our documentation.

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